Cell signaling, the process by which cells respond to external stimuli in their environment, occurs in yeast cells via mechanisms that are remarkably similar to those employed by more complex eukaryotes, or multicellular organisms, such as humans. Understanding this fundamental process may lead to breakthrough discoveries in the quest to find cures for various cancers. Various bio-chemical methods and the two-hybrid assay have been used to further define the molecular mechanisms by which profilin, an actin binding protein, contributes to actin regulation in yeast. Two-hybrid studies identified interactions of profilin with adenylyl-associated protein (CAP/Srv2p), which is also an actin binding protein. In vitro bio-chemical analyses suggest that the interaction between profilin and CAP/Srv2p is direct, while the profilin and adenylyl cyclase interaction is indirect and may be mediated by CAP/Srv2p. Due to the similarity of cell signaling processes in yeast and human cells, determining the interaction of these proteins with their external environment will give us a better understanding of how cancer cells respond to external stimuli, and give clues as to what may be done to halt their profuse division.